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KMID : 0378019710140110099
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New Medical Journal
1971 Volume.14 No. 11 p.99 ~ p.107
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An Autoradiographic Study of Effects of DL-Ethionine on Protein Synthesis of Pancreas of Mice
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Abstract
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Seventy-two young Balb C/Strain, having an average weight of 20gm., were maintained on a standard laboratory diet.
They were divided into six groups in according to DL-ethionine doses.
Group I acted as a control group. Group i , fl , IV, V , and Vl were given 100 mg, 200 mg, 400 mg, 600 mg and 900 mg of DL-ethionine, respectively, by the intraperitoneal route.
Following the last injection, each groups were sacrificed on days 1, 3, 5 and 10.
Thirty minutes prior to sacrifice 5pc/gm body weight of Phenylalanine-C14 (specific activity 250 mc/ mM) were injected. Piece of pancreas from each animal were fixed in 2% paraformaldehyde in cacodylate buffer and double embedded in parlodion paraffin and sectioned at 4,u and stained with toluidine blue o.
The effects of DL-ethionine administration upon protein synthesis in the pancreas of mice were investig￢ated with light microscopic radioautography.
1. Zymogen degranulation and a loss of basal basophilia in the acinous portion of the pancreas were observed after injection of 400mg DL-ethionine.
In the 600mg DL-ethionine injection inflammation, edema, necrosis were evident. Marked fibrosis was evident when 900mg DL-ethionine was injected. No cytological changes were observed in the islands of Langerhans.
2. The number of grains per cell in the experimental animals was significantly lower on day 5 after injection of the 900 mg DL-ethionine.
3. The result is interpreted to indicate that overall protein synthesis in pancreas is immediately suppressed by DL-ethionine and that such suppression might lead to a later functional disturbance among these cells.
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